Label-free hematology analysis using deep-ultraviolet microscopy

DeadNet: Identifying Phototoxicity from Label-free Microscopy Images of Cells using Deep ConvNets

Exposure to intense illumination light is an unavoidable consequence of fluorescence microscopy, and poses a risk to the health of the sample in every live-cell fluorescence microscopy experiment. Furthermore, the possible sideeffects of phototoxicity on the scientific conclusions that are drawn from an imaging experiment are often unaccounted for. Previously, controlling for phototoxicity in i...

Label-free detection of single protein molecules using deep UV fluorescence lifetime microscopy.

We present the detection of single beta-galactosidase molecules from Escherichia coli (Ecbeta Gal) using deep UV laser-based fluorescence lifetime microscopy. The native fluorescence from intrinsic tryptophan emission has been observed after one-photon excitation at 266 nm. Applying the time-resolved single-photon counting method, we investigated the fluorescence lifetime distribution and the b...

Label-Free Detection of Breast Masses Using Multiphoton Microscopy

Histopathology forms the gold standard for the diagnosis of breast cancer. Multiphoton microscopy (MPM) has been proposed to be a potentially powerful adjunct to current histopathological techniques. A label-free imaging based on two- photon excited fluorescence and second-harmonic generation is developed for differentiating normal breast tissues, benign, as well as breast cancer tissues. Human...

Label-free monitoring of colonic cancer progression using multiphoton microscopy

Real-time histology or virtual biopsy for the diagnosis of colonic cancer is of great medical significance. In this work, we show that label-free multiphoton imaging is feasible and effective in monitoring colonic cancer progression by providing cellular and subcellular details in fresh, unfixed, unstained colonic specimens. Our results also demonstrate the capability of using tissue quantitati...

Label-Free Quantitative Analysis Using LC/MS